![]() This may disrupt the breakdown and absorption of nutrients, and affect the growth and action of intestinal flora. Lectins can also bind to cells lining the digestive tract. Legumes and cereals often contain these minerals, so the concurrent presence of lectins may prevent the absorption and use of these minerals in the body. Milder side effects include bloating and gas.Īnimal and cell studies have found that active lectins can interfere with the absorption of minerals, especially calcium, iron, phosphorus, and zinc. It can also produce nausea, vomiting, stomach upset, and diarrhea. They contain phytohaemagglutinin, a type of lectin that can cause red blood cells to clump together. The most publicized accounts report severe reactions in people eating even small amounts of raw or undercooked kidney beans. When consumed, lectins in their active state can cause negative side effects. They resist being broken down in the gut and are stable in acidic environments, features that protect lectin-containing plants in nature. The same features that lectins use to defend plants in nature may cause problems during human digestion. ![]() ![]() Lectins are defined as proteins that bind to carbohydrates. Is there truth behind these claims? The Problem With Lectins They are found in all plants, but raw legumes (beans, lentils, peas, soybeans, peanuts) and whole grains like wheat contain the highest amounts of lectins. Lectins, or hemagglutinins, are an “ anti-nutrient” that have received much attention due to popular media and fad diet books citing lectins as a major cause for obesity, chronic inflammation, and autoimmune diseases. ![]()
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![]() ![]() Nicole: We genuinely love the look of the yurt! Our living space has such a relaxing and geometric look to it. We felt so much accomplishment and joy when we were able to sleep in our new yurt on night one! It also made us feel very accomplished, and we found the structure itself to be designed so mindfully. I must say, Nicole really enjoyed the process of putting up the yurt because it went by so fast. After clearing the space, we built the platform and assembled the yurt in about a month–despite the snow, rain, black flies, and mosquitos attempting to stop us! Once the platform was finished, we waited for a stretch of sunny weather to happen, which let us put up our yurt in just three days. We also had to cut our way into our land and clear a spot for the yurt because our property was so heavily forested and raw. Jake: It’s been a fun and challenging adventure! Our property can only be accessed by boat, so our materials had to be barged over to us. This was our new home! What’s it like living in an off the grid yurt? Seeing the French doors, the glass windows, the frames for the windows and the dome brought tears to my eyes. Unwrapping each piece was like opening a Christmas present for us. However, when we started to put the Yurt together, it was a blast. In fact, it took us a lot longer to finish the platform because we were building the platform over three feet high on very uneven ground. I would say the most challenging part was building the yurt platform. Nicole: The assembly process had its moments. We just knew their helpfulness and professionalism with our situation would carry over into their yurt design! Can you talk about the assembly process and what that’s been like? ![]() We loved being able to use the Pacific Yurts 3D Yurt Builder on their website to customize a yurt and see what ours would look like!įinally, of all the companies we looked at, Pacific Yurts was the most helpful and responsive to our questions and our ideas when we communicated with them over email or by phone. We were also impressed by Pacific Yurts’ professional social media pages, which allowed us to see a wide variety of ways their yurts were built and lived in among hundreds of different settings. We kept coming back to their yurts because they looked very clean, resilient, and mindfully designed around the structure’s traditions, but with added modern features. Jake: We researched about 10 other yurt companies before choosing Pacific Yurts. We relished the chance to design our own yurt interior, so we thought a Pacific Yurt would be the perfect dwelling for our move to raw forested property in Canada! What made Pacific Yurts standout over other structures or companies on the market? Many of the families we saw living in Yurts had these amazing living spaces that looked comfortable and quite beautiful. Nicole and I also enjoy watching YouTube videos of families and couples who build and live in unique housing. While in the grasslands of Mongolia, I stayed in a traditional Mongolian Yurt – it was an amazing experience! I remember loving the design and feeling of warmth and comfort that the yurt gave me while I was wrapped in wool blankets safe inside that Mongolian Yurt. Jake : I traveled throughout China and Mongolia when I was 21 years old. What inspired you to pursue an off the grid yurt lifestyle? W e live in a 30 foot Pacific Yurt located on North Vancouver Island. Let’s start with a quick introduction of you and your yurt. To learn more about their adventures, we sent them a Q&A to see what it’s like to live off the grid in a Pacific Yurt. We’ve since launched a video series covering their incredible journey that takes viewers through their process of building a yurt and living in the ruggedness of Canada’s wilderness. They also told us they wanted to share their story about the benefits of living in a yurt by bringing more awareness to off the grid yurt living. ![]() When Jake and Nicole visited our facility to pick up their Pacific Yurt, they told us they wanted to start a sustainable property centered around a yurt. ![]() ![]() ![]() Try using the barbed wire pattern here, the sharp edges and dark lines stand out very well. The Grim Reaper tattoos in the tribal art pattern look very cool. Car accident victims may wear this tattoo for the same reason. Tribal Grim Reaper Tattoo designs serve as a reminder for someone who has kicked an addiction that they may not cheat death next time. You will find that in the tattoo world, there are many symbolic reasons why people wear Grim Reaper tattoos. Some may see them as the “evil death is near symbol” with which they are commonly associated. Tribal Grim Reaper tattoos are just as popular nowadays as they have been in the past. This is why we often see the Grim Reaper in pop culture doing silly or human things, which also reflect in Grim Reaper tattoo designs such as a Grim Ripper is riding a skateboard or smoking a blunt. The perpetrator of the greatest injustice in the universe is that we all have to die. Jokes are funny when we aim them at a perpetrator of injustice. ![]() Grim Reaper tattoos are a great reminder of that. An image like the Grim Reaper is a moral assurance that death is fair. Like many religious or spiritual icons, personifications of death help to cope with a complicated, scary, and mysterious idea of death. This also reflects in a lot of Grim Reaper Tattoo designs because people love to laugh in the face of death.Įvery culture deals with death in their own way. If you look at The New Yorker’s cartoons that featured the Grim Reaper, then you will notice that he’s always the butt of the joke. In addition to his many appearances on late-night TV, early morning cartoons, and movies, the Grim Reaper has appeared in over hundreds of animations since 1937. The Grim Reaper has been a cultural icon for decades. Grim Reaper tattoos are a representation of death. Death is most often personified in male form, although in some cultures, death is a female. Grim Reaper is also known as the Angel of Death. In some mythologies, the Grim Reaper causes death by collecting humans’ souls. Grim Reaper is a personification of death depicted in the form of a cloaked skeleton wielding a large scythe. One of the most common and enduring of all is the Grim Reaper. You’re satisfied, press “set as wallpaper.” 8.For thousands of years, various cultures have had figures to represent death. You’ll then be able to move the image to how you like. You’ll then be prompted to select whether you want to set the image as the background of Right corner, click the menu button (three vertical dots). The first image you see here should be the image you downloaded. Open your gallery/photos app and click on the “download” folder.ģ. Search for a wallpaper you like on and download it clicking on the blueĭownload button below the wallpaper. Navigate back to your home screen and take a look at your new wallpaper. Whether you want this image to be set as the background of your lock screen, home screen or both. Here you can arrange the picture how you want it, then tap “set.” 8. (the one that looks like a box with an arrow coming out of it). Navigate to the “Photos” app and find the image you want as your background. Tap on an image and hold on a few seconds. Find an image you like on and click on the blue download button Exit back to your desktop and see what it looks like! iPhone/iPad 1. Here you’ll want to select your own, so you’ll select the location your new imageĭownloaded. This order, click Apple Menu > System Preferences > Desktop & Screen Saver > Desktop 3. Download your favourite wallpaper clicking on the blue download button below the The folder and click “Set as desktop background.” 6.Enjoy your new wallpaper! Mac 1. That image on your computer (it will probably be in your “downloads” folder) 5.Right-click the image in That part for you.) 3.Click the button, and you’ll notice the image save to your browser. That says “Free Download.” Just below that text is your screen’s resolution (don’t worry, we calculated First, find the perfect wallpaper for your PC. ![]() ![]() ![]() Place the blot in destain solution and agitate for 5 to 10 minutes to remove excess stain. Stain: 0.1% (w/v) amido black in 25% (v/v) isopropanol, 10% (v/v) acetic acidĭestain: 25% (v/v) isopropanol, 10% (v/v) acetic acidĪgitate your blot in the stain for 10 minutes, rinse with distilled water. ![]() The dye will wash out completely.Īn extra tidbit: After you have done your Western blot visualization, stain the blot with amido black: After you’ve seen your proteins have transferred, continue to rinse, or go straight into a blocking step. Agitate your blot in the stain for 2-5 minutes, rinse with distilled water. ![]() There are several variations of the recipe out there, the most simple being 0.1% w/v Ponceau-S in 5% acetic acid. One of the most basic checks for protein transfer: Ponceau-S staining. You can also try staining the second membrane with Coommassie blue, which will show you all of the proteins that have transferred to that layer, and not just your protein of interest.įor more information on these transfer controls, check out this video from Agrisera. This is more likely to occur to the smallest proteins first so pay particular attention to where the lower molecular weight ladder bands should appear. If you are transferring too long, you will detect protein on the second membrane as well. If your transfer conditions are good, then the majority of your protein will be on the first membrane (closest to the gel). Then, go ahead and blot both membranes (be sure to label which was farthest away from the gel!). To test is your transfer is too long, try inserting two membranes into your transfer stack, one right behind the other. The two methods described above are most useful for determining whether the transfer duration was too short. So remember to do this test before you run your precious samples! Transfer your gel onto two membranes Instead, you’ll need to run a second gel and do the transfer again using the adjusted conditions. Again, this is a “post-hoc” check for transfer efficiency: once you’ve stained the gel, you won’t be able to set it up for a second transfer. This is a quick and easy procedure that will give you a good idea of how much protein is still in the gel: if the gel is mostly clear, than your transfer was successful, but if most of your gel shows blue bands, then your transfer needs to run longer. ![]() After transfer, stain the SDS-PAGE gel with Coommassie blueĪnother simple way to monitor the efficiency of protein transfer is to stain the gel with Coommassie after your transfer is complete. Remember, if you’ve opened your transfer cassette to “peek” at the ladder, this will introduce air bubbles to the transfer stack – so you probably want to use this technique to optimize transfer times when you’re not running important samples. If you use a ladder where each band is stained a different color, this can help you keep track of the transfer efficiency of differently sized proteins. Instead, you are aiming for a point at which most of the larger proteins have migrated to the membrane, and most of the smaller proteins are still on the membrane but not through it. Keep in mind that proteins of different sizes will migrate at different speeds: there is no magic moment at which all the proteins jump from the gel to the membrane. The rest of the proteins on your gel will of course be invisible, but you can easily see if the brightly colored ladder bands are still in the gel, or have transferred completely to the membrane. Use a pre-stained protein ladder to track transfer of proteins from a gel to a membrane. So how do you know when your transfer is complete? Here are a few good controls you can use to monitor transfer efficiency: Look for complete transfer of the molecular weight ladder Instead, you will need to optimize the transfer time and conditions for yourself, based on the equipment you use and the proteins you are working with. Transfer times are empirical and based on your own particular samples, which means that there is no easy way to determine how long you need to run the transfer in order to get complete transfer of all your proteins. Now it’s time for the all-important transfer step, that tricky point that will determine the quality of your Western blot. So, you’ve done your experiment, prepped your samples, and run your SDS-PAGE gel. ![]() ![]() ![]() ![]() The court will not appoint an attorney for a party in a civil (non-criminal) case in General District Court.ĭo I need an attorney to bring a lawsuit in General District Court? Will the court appoint an attorney for me? You also may ask for a later trial date and time. If a Counterclaim is filed against you and is not a detailed explanation, you may ask the Judge to order the other party to file a Bill of Particulars. It is a claim that you owe money or property to the party you have sued. If you don't file by this date, your lawsuit may be dismissed.Ī "Counterclaim" is a claim that the other party files against you. You also will have a date by which to file your Bill of Particulars. ![]() If the Judge orders you to file a Bill of Particulars, you will have a later trial date and time. It is a more complete explanation of why you should get the money or property you are asking for. The case could be heard and tried right then.Ī "Bill of Particulars" is a written statement giving details of the lawsuit.The case could be set for a later trial date and time.The other party could file a Counterclaim against you.The other party could ask the Judge to order you to file a "Bill of Particulars.".In Circuit Court, the case will be removed to Circuit Court. If your claim involves more than $4,500 and the other party wants the case to be heard.You will have a later trial date and time in another General District Court. Heard elsewhere, the case will be transferred to the proper General District Court. If you filed in the wrong General District Court and the other party wants the case to be.This means you will have a later trial date and time. If you filed in the Small Claims Division and the other party wants an attorney, theĬase will be removed to the regular General District Court.What usually happens at the first court hearing?Īt the first court hearing, the following things can happen. Planning for and Proving Your Lawsuit. How to Take a Civil (Non-Criminal) Lawsuit to General District Court (Part 2 of 3) ![]() ![]() ![]() The Dragonbone war axe, bow, and maul are standouts for being the best non-legendary weapons in their categories, and also being cheap to make. The standout best weapons, the Teith's swords, are craftable and hardened steel, and the Kingslayer spear is so close to being the top you might as well ignore the legendaries here. In general the end game gear is fucked, more weapons than armor though. This would encourage people to use other gear, and take away most of the competitive edge that players get by rushing this crap.Ī post writing preface: this turned into a bit of a rant. The armor should be cut down by about 10-15% and the stats should be brought down to +2. I think with the considerations above in mind that it would make sense to nerf this set a bit. I do concede that if you're going for an optimal build, and/ or 50 points in a stat then it's best to use other armor. If someone doesn't go heavy into one stat then the disadvantages of using this set is negligible. The division of stats on Silent Legion gear is not optimal, but it's still not terrible. The fact that most people use it is a testament to it's overall power.Īre there better or more optimal armor sets? Absolutely. It provides a ridiculous amount of armor, heat protection, and doesn't require a good/ rare thrall to create. No, it's blatantly too powerful for the low requirements to get the blueprint and craft.
![]() ![]() Of course, it is not the same as actually taking a slave into the store and humiliating him live in front of the pretty sales girls, but it's still very hot - with the right man. I've developed many lingerie store humiliation tasks over the last few years. Or, they are not members of my site, but still long for the challenge and humiliation of the Victoria's Secret task. Many sluts, though, follow my lessons in private and want to attempt this task. Sass up your sissy lifestyle! Click here to shop our curated collection of BDSM accessories and sexy lingerie for sissies.Sissies that are enrolled and in good standing in my training (that means ALL OF YOUR SLUT HOMEWORK is turned in) will receive this task as part of their training. Looking for more daily tasks and assignments for sissies? Click here to purchase our E-book, 6 Weeks of Sissy Tasks! A reminder that any BDSM activity, including humiliation, requires consent, negotiation, safety and preparation. Ultimately, sissification can be thrilling, satisfying and enjoyable for a diverse and personal array of reasons. Do a handstand against the wall, upside down, legs in the air, and urinate.Once they are all in place, take a video slapping them off one by one. Put at least 10 clamps on your body (both nipples, clitty, tongue, nostrils, etc.Use the dildo to make yourself c*m, and send a video of the experience. ![]() Stick a dildo with a suction cup somewhere in your house and suck it.Go swimming in a public pool wearing a bikini.Get a pink glitter mani/pedi at a nail salon.Write a letter apologizing for being a sissy + post it online.Stay locked in chastity for a month + send daily photos.Book a 60-minute, full-face appointment at Sephora and get a glam makeover.(Tight, thick nylon/lycra work best for noticeability.) ![]() Wear tight pants/shorts in public + ensure panty lines are visible.Now that we've covered the basics, keep reading for a list of unique and creative activities for our beloved sissies from mild to…very experimental! Neurochemical response: humiliation can trigger a neurochemical response that releases endorphins, similar to a "runner's high." This can lead to feelings of euphoria or pleasure afterward.For some "sissies," the act of trusting someone to humiliate them-and being cared for afterward-can result in deep feelings of intimacy and connection. Trust and Intimacy: engaging in humiliation tasks requires substantial trust between participants.Believe it or not, humiliation is one way to help process feelings of guilt, shame, or other emotions in a controlled environment. Catharsis: experiencing humiliation can be cathartic for some individuals.Being given tasks and performing them might reinforce their role and self-image. Validation: for some "sissies," humiliation tasks can serve as a form of validation for their submissive or feminized identity.Eroticized shame: associations of shame and/or embarrassment can evolve into a desire for humiliation tasks in adulthood as a way to relive those emotions, and find pleasure in former shameful experiences.Releasing control, submitting to the Dominant partner or a chastity keyholder, and performing these tasks, are often known to relieve everyday stress and/or societal expectations. The great escape: humiliation tasks can provide an escape from their daily life. ![]() Humiliation tasks, in this context, might be a way for the dominant person to exert control over the submissive, reinforcing the power differential.
![]() ![]() Increases the Level of Rites of Termination by 3. ![]() Ravaging Confession's CRIT Hits regenerate 5 Energy for Rosaria.Ĭan only be triggered once each time Ravaging Confession is cast. Increases the Level of Ravaging Confession by 3. The duration of the Ice Lance created by Rites of Termination is increased by 4s. When Rosaria deals a CRIT Hit, her ATK SPD increases by 10% and her Normal Attack DMG increases by 10% for 4s. Skill Effect: Increases Elemental Burst DMG by 16% and Elemental Burst CRIT Rate by 6%.Īll Recommended Weapons for Rosaria Recommended Weapons If there are less than 2 enemies nearby, ATK is increased by 24%.īest Free-to-Play Weapon for Rosaria Weapon Skill Effect: If there are at least 2 enemies nearby, ATK and DEF is increased by 16%. When the wielder's HP is less than 50%, this ATK Bonus is increased by an additional 1% of Max HP. Additionally, provides an ATK Bonus based on 0.8% of the wielder’s Max HP. While in possession of the maximum possible stacks, DMG dealt is increased by 12%. This effect can only occur once every 0.3s. Skill Effect: On hit, increases ATK by 3.2% for 6s. List of Artifacts and Set Bonuses Rosaria Best Weapons Top 3 Rosaria Weapons When HP is below 70%, CRIT Rate increases by an additional 24%.A maximum of 75% bonus DMG can be obtained in this way. Increases Elemental Burst DMG by 25% of Energy Recharge.If the enemy is Frozen, CRIT Rate is increased by an additional 20%. When a character attacks an enemy affected by Cryo, their CRIT Rate is increased by 20%.Using an Elemental Burst increases all party members' ATK by 20% for 12s.Best Artifacts for Rosaria Best Artifacts Ranked Rating If she's just a Sub-DPS, focus on her Elemental Skill after her Burst instead. Rosaria's Elemental Burst will be used very often in any role, so prioritize leveling it, along with her Normal Attacks if you're using her as a Main DPS. Other than ensuring you have enough Energy Recharge to burst off-cooldown, try to reach 100% CRIT Rate to provide the team a 15% CRIT buff! Rosaria's Talent Priority This Support build for Rosaria is all about providing as many buffs to her team as possible. Energy Recharge, CRIT Rate, CRIT DMG, ATK% ![]() ![]() ![]() It’s a great way try Afraid Of The Dark before you buy it! It contains 20 patches from the original library and even though the patches have somewhat limited functionality compared to the full library, there are some great sounds here that many composers and dark ambient artists will absolutely love. Indefinable Audio – Afraid Of The Dark FREE // Free version of Afraid Of The Dark for Omnisphere 2.Airwave 20 Year Omnisphere Power Pack // A smaller collection of the Airwave 20 years Power Pack for Omnisphere created by Belgian DJ and Producer Airwave.Third Eye by StudioWavKits // 20 Amazing Trap Presets to make some Cubeatz, Murda, Travis Scott Type Samples.Monstars by StudioTrap // 5 Free custom preset patches influenced by today’s top urban artists and producers such as Juice Wrld, Gunna, 21 Savage, Da Baby, Future, Travis Scott, and many more.While the Premium Kit includes 52 Patches, the free kit includes 7 Free patches influenced by artists and producers like Juice Wrld, Future, Travis Scott, Da Baby, 808 Mafia, Drake and many more. Versace Free Presets //Free sample download of the ‘Versace’ Premium Omnisphere Bank.Free Omnisphere Bank Lift Off by Loop Legendz // 20 Free Top-Notch Presets inspired by Drake, The Weeknd, Travis Scott and more….Lost Free Bank by Loop Legendz // 25 Custom Made Omnisphere 2 Presets including Arps, Bells, Leads, Pads, Pluck, String, Texture and even a trumpet lol.Enter The Space Age //10 Amazing custom presets including bass, Arps, flute, pad, and synth! A must-have for any hip-hop producer!.Vengeance Free Omni Bank by Dj Shawdi P // It includes custom Arps, Keys, Leads, Pads, Plucks & More inspired by current industry producers. ![]() Pharoah Free Omnisphere Preset Bank // Contains 50 custom presets including Arps, Bells, Choir, Synth, Keys, Leads, Pads, Plucks, Pianos, FX inspired by Playboi Carti, Future, Lil Pump, Trippie Red, Lil Uzi Vert & More! Perfect for making spacey New Wave Trap Beats.The Free Pack comes with a variety of Keys, Leads, Pads, Bell Pads, Synths, Flutes & Bass presets. Producer Grind Grind Umbrella Preset Bank // Our friends at Producergrind have made an incredible job creating 45 dope trap Presets.This has a wide variety sounds including Arps, plucks, pads, synths & everything in between. Shout out to for submitting this dope pack to drop on the community for FREE. Soul Fusion Bank by MoePresets // 50 original Omnisphere 2 presets for making beats. ![]()
![]() ![]() Realtor Upton MA | Real Estate Agent Upton MassachusettsĪre you going to be changing your address? An address change with the USPS is a regular part of a home buyer or seller’s routine.Realtor Westborough MA | Top Real Estate Agent Westborough Mass.Realtor Southborough MA | Real Estate Agents Southborough Mass.Realtors Shrewsbury Mass | Real Estate Guide Shrewsbury.Realtor Northbridge MA | Real Estate Agents Northbridge.Realtor Millbury MA | Top Real Estate Agent Millbury Mass. ![]()
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